Hotspot

rps8-rpl14

LSC · 86523-86715 · 193 bp

Back to Cyclea

Pi 0.0363
MarkerSeek score 73.40
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
193
variable_sites
7
pi
0.0363
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 TCAGCAATAGTGTCTTTACCCA GCTCGGGAATTGAGACAGTT 40.9 50.0 57.1 57.9 279 bp 1.0 58.0
rps8-rpl14_p2 TCAGCAATAGTGTCTTTACCCA AAACCCAAAAGGAACTCGGG 40.9 50.0 57.1 58.3 313 bp 1.0 56.2
rps8-rpl14_p3 TCAGCAATAGTGTCTTTACCCA CCCAAAAGGAACTCGGGTTT 40.9 50.0 57.1 58.3 310 bp 1.0 56.2
rps8-rpl14_p4 TCAGCAATAGTGTCTTTACCCA AGGAACTCGGGTTTTTGGTG 40.9 50.0 57.1 58.3 304 bp 1.0 56.1
rps8-rpl14_p5 TCCATGTCAGCATTTCGTAT GCTCGGGAATTGAGACAGTT 40.0 50.0 54.8 57.9 312 bp 1.0 55.7