Hotspot

atpB-rbcL

LSC · 59584-60403 · 820 bp

Back to Cyclea

Pi 0.00734
MarkerSeek score 63.30
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
820
variable_sites
6
pi
0.00734
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 AAACCCCAGGACCAGAAGTA AATGCTGCTAGCGTATCAGT 50.0 45.0 57.9 57.1 974-976 bp 1.0 72.5
atpB-rbcL_p2 ACTACATCCAGTACCGGACC AATGCTGCTAGCGTATCAGT 55.0 45.0 58.2 57.1 1038-1040 bp 1.0 71.7
atpB-rbcL_p3 CTACATCCAGTACCGGACCA AATGCTGCTAGCGTATCAGT 55.0 45.0 58.2 57.1 1037-1039 bp 1.0 71.7
atpB-rbcL_p4 TACATCCAGTACCGGACCAA AATGCTGCTAGCGTATCAGT 50.0 45.0 57.8 57.1 1036-1038 bp 1.0 71.6
atpB-rbcL_p5 AAACCCCAGGACCAGAAGTA CACCAGCTTTGAATCCAACAC 50.0 47.6 57.9 58.0 904-906 bp 1.0 71.6