Hotspot

ndhC-trnV-UAC

LSC · 55258-56500 · 1243 bp

Back to Cyclea

Pi 0.00414
MarkerSeek score 63.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1243
variable_sites
5
pi
0.00414
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTTTTCTGGTCCTTCGCGAA TGGGTCCTTGAAACAGTTCG 45.0 50.0 58.0 58.0 1432-1454 bp 1.0 81.6
ndhC-trnV-UAC_p2 TTTTTCTGGTCCTTCGCGAA TGCATGTTGGGTCCTTGAAA 45.0 45.0 58.0 57.9 1439-1461 bp 1.0 81.2
ndhC-trnV-UAC_p3 TTTCTGGTCCTTCGCGAATT TGGGTCCTTGAAACAGTTCG 45.0 50.0 57.8 58.0 1430-1452 bp 1.0 80.5
ndhC-trnV-UAC_p4 TTTTCTGGTCCTTCGCGAAT TGGGTCCTTGAAACAGTTCG 45.0 50.0 57.8 58.0 1431-1453 bp 1.0 80.5
ndhC-trnV-UAC_p5 TTTTTCTGGTCCTTCGCGAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 58.0 57.8 1374-1396 bp 1.0 80.4