Hotspot

psbZ-trnG-GCC

LSC · 39721-40446 · 726 bp

Back to Cyclea

Pi 0.0139
MarkerSeek score 67.70
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
726
variable_sites
10
pi
0.0139
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG AATCGAACCCGCATCTTCTC 50.0 50.0 57.8 58.1 894 bp 1.0 79.4
psbZ-trnG-GCC_p2 TTGCTTCTCCTGATGGTTGG GAATCGAACCCGCATCTTCT 50.0 50.0 57.8 58.1 895 bp 1.0 79.4
psbZ-trnG-GCC_p3 GCTTCTCCTGATGGTTGGTC AATCGAACCCGCATCTTCTC 55.0 50.0 58.3 58.1 892 bp 1.0 79.0
psbZ-trnG-GCC_p4 GCTTCTCCTGATGGTTGGTC GAATCGAACCCGCATCTTCT 55.0 50.0 58.3 58.1 893 bp 1.0 79.0
psbZ-trnG-GCC_p5 TTGCTTCTCCTGATGGTTGG GAACCCGCATCTTCTCCTTG 50.0 55.0 57.8 58.6 890 bp 1.0 74.9