Hotspot

trnD-GUC-trnY-GUA

LSC · 32989-33524 · 536 bp

Back to Cyclea

Pi 0.0174
MarkerSeek score 70.60
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
536
variable_sites
9
pi
0.0174
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GTGCTCTGACCGATTGAACT TCTACGCTGGTTCAAATCCG 50.0 50.0 57.9 58.0 578-594 bp 1.0 83.0
trnD-GUC-trnY-GUA_p2 GTGCTCTGACCGATTGAACT ATGGATTCTTCCTGGGTCGA 50.0 50.0 57.9 58.1 645-661 bp 1.0 81.7
trnD-GUC-trnY-GUA_p3 GTGCTCTGACCGATTGAACT TGGATTCTTCCTGGGTCGAT 50.0 50.0 57.9 58.1 644-660 bp 1.0 81.7
trnD-GUC-trnY-GUA_p4 AATCCTAGGTCGGGACTGAC TCTACGCTGGTTCAAATCCG 55.0 50.0 58.2 58.0 634-650 bp 1.0 81.6
trnD-GUC-trnY-GUA_p5 CAATCCTAGGTCGGGACTGA TCTACGCTGGTTCAAATCCG 55.0 50.0 58.2 58.0 635-651 bp 1.0 81.5