Hotspot

rpoB-trnC-GCA

LSC · 28169-29467 · 1299 bp

Back to Cyclea

Pi 0.01
MarkerSeek score 62.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1299
variable_sites
13
pi
0.01
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AAGTTCTTCGATCAAGCCCC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.9 58.1 1466-1480 bp 1.0 79.2
rpoB-trnC-GCA_p2 TCAATGAACCCACGGAATCC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.9 58.1 1444-1458 bp 1.0 79.2
rpoB-trnC-GCA_p3 CCCACGGAATCCCTCAAATT CCGGATTTGAACTGGGGAAA 50.0 50.0 57.9 58.1 1436-1450 bp 1.0 79.0
rpoB-trnC-GCA_p4 TCAAGCCCCGATCAATGAAC CCGGATTTGAACTGGGGAAA 50.0 50.0 58.2 58.1 1455-1469 bp 1.0 78.2
rpoB-trnC-GCA_p5 GATCAAGCCCCGATCAATGA CCGGATTTGAACTGGGGAAA 50.0 50.0 57.7 58.1 1457-1471 bp 1.0 78.1