Hotspot

trnK-UUU-rps16

LSC · 4793-5676 · 884 bp

Back to Cyclea

Pi 0.00792
MarkerSeek score 63.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
884
variable_sites
7
pi
0.00792
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTACGGACGAATCCATTGC TCCTTGAAAAAGGTGCCCAA 50.0 45.0 58.1 57.8 1093-1117 bp 1.0 78.7
trnK-UUU-rps16_p2 AGTACGGACGAATCCATTGC TTCCTTGAAAAAGGTGCCCA 50.0 45.0 58.1 57.8 1094-1118 bp 1.0 78.7
trnK-UUU-rps16_p3 GTACGGACGAATCCATTGCT TCCTTGAAAAAGGTGCCCAA 50.0 45.0 58.1 57.8 1092-1116 bp 1.0 78.7
trnK-UUU-rps16_p4 GTACGGACGAATCCATTGCT TTCCTTGAAAAAGGTGCCCA 50.0 45.0 58.1 57.8 1093-1117 bp 1.0 78.7
trnK-UUU-rps16_p5 GTACGGACGAATCCATTGCT CCTTGAAAAAGGTGCCCAAC 50.0 50.0 58.1 57.8 1091-1115 bp 1.0 78.1