Hotspot

psbA-trnK-UUU

LSC · 1743-2178 · 436 bp

Back to Cyclea

Pi 0.0161
MarkerSeek score 62.80
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
436
variable_sites
7
pi
0.0161
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 TGTACAGACGGTTTTCGGTG GTTTGGGGAGGGGTTTTTCT 50.0 50.0 58.1 57.9 626-628 bp 1.0 79.2
psbA-trnK-UUU_p2 TGTACAGACGGTTTTCGGTG CTAGTTCCGGGTTCGAATCC 50.0 55.0 58.1 57.8 554-555 bp 1.0 78.2
psbA-trnK-UUU_p3 TGTACAGACGGTTTTCGGTG TTTGGGGAGGGGTTTTTCTC 50.0 50.0 58.1 57.6 625-627 bp 1.0 76.9
psbA-trnK-UUU_p4 TGTACAGACGGTTTTCGGTG AAAATGCAAGCACGGTTTGG 50.0 45.0 58.1 58.4 640-642 bp 1.0 76.6
psbA-trnK-UUU_p5 TTTTCGGTGCTGGTTATCCA GTTTGGGGAGGGGTTTTTCT 45.0 50.0 57.4 57.9 615-617 bp 1.0 75.8