Hotspot

ycf1

SSC · 121791-127313 · 5523 bp

Back to Cyclamen

Pi 0.0139

MarkerSeek score 49.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5523
variable_sites: 141
pi: 0.0139
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCACCTTTGATCTAAGATCTCT	TGCATATAAAATGAAACCCTGGT	39.1	34.8	57.1	56.5	201-207 bp	1.0	43.9
ycf1_p2	GGTTTTGCACCTTTGATCTAAGA	TGCATATAAAATGAAACCCTGGT	39.1	34.8	57.3	56.5	206-212 bp	1.0	43.8
ycf1_p3	GGTTTTGCACCTTTGATCTAAGA	TGCATATAAAATGAAACCCTGGTT	39.1	33.3	57.3	57.1	206-212 bp	1.0	43.8
ycf1_p4	GGTTTTGCACCTTTGATCTAAGA	CTTTGGTTCTTCCCAGAATT	39.1	40.0	57.3	53.3	241-247 bp	1.0	43.3
ycf1_p5	GGTTTTGCACCTTTGATCTAAGA	CTTTGGTTCTTCCCAGAATTT	39.1	38.1	57.3	54.2	241-247 bp	1.0	43.3