Hotspot

rps15

SSC · 121113-121385 · 273 bp

Back to Cyclamen

Pi 0.00794

MarkerSeek score 43.10

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 4
pi: 0.00794
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	GAGGTCCTTTCTTGTAGCCG	CCCACAATTGAAAAGCGCTT	55.0	45.0	58.0	57.8	780-851 bp	1.0	86.2
rps15_p2	GAGGTCCTTTCTTGTAGCCG	ATGGCAGTCGTTTCAGTCTG	55.0	50.0	58.0	58.2	817-888 bp	1.0	85.7
rps15_p3	AACACCATGCATTGATGGGT	CCCACAATTGAAAAGCGCTT	45.0	45.0	58.1	57.8	825-896 bp	1.0	85.7
rps15_p4	GAAAGGAGTCTGTGCACCAA	CCCACAATTGAAAAGCGCTT	50.0	45.0	58.0	57.8	1143-1214 bp	1.0	84.7
rps15_p5	TCTGTGCACCAATATCTGCC	CCCACAATTGAAAAGCGCTT	50.0	45.0	58.0	57.8	1135-1206 bp	1.0	84.7