Hotspot

ndhF

SSC · 107960-110176 · 2217 bp

Back to Cyclamen

Pi 0.0139

MarkerSeek score 52.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 58
pi: 0.0139
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	TTCTGCTTGTGCTGTATCGA	50.0	45.0	58.0	57.3	2715-2733 bp	1.0	77.1
ndhF_p2	AAGAGGGATCCACCGAAGAA	TTCTGCTTGTGCTGTATCGA	50.0	45.0	58.0	57.3	2707-2725 bp	1.0	77.1
ndhF_p3	GCAGAATACCGTCACCCATT	TTCTGCTTGTGCTGTATCGA	50.0	45.0	58.0	57.3	2812-2830 bp	1.0	77.0
ndhF_p4	TGAAACGAAAGAGAGCCGAG	TTCTGCTTGTGCTGTATCGA	50.0	45.0	57.9	57.3	2634-2652 bp	1.0	77.0
ndhF_p5	GAAACGAAAGAGAGCCGAGT	TTCTGCTTGTGCTGTATCGA	50.0	45.0	57.9	57.3	2633-2651 bp	1.0	77.0