Hotspot

ndhH-rps15

SSC · 121037-121112 · 76 bp

Back to Cyclamen

Pi 0.0965

MarkerSeek score 71.50

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 76
variable_sites: 12
pi: 0.0965
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	GAGGTCCTTTCTTGTAGCCG	GAAAATTCTGGGAAAGCGCC	55.0	50.0	58.0	58.0	218-249 bp	1.0	84.8
ndhH-rps15_p2	GAGGTCCTTTCTTGTAGCCG	GCGGAAAATTCTGGGAAAGC	55.0	50.0	58.0	58.0	221-252 bp	1.0	84.8
ndhH-rps15_p3	GAGGTCCTTTCTTGTAGCCG	GGAAAATTCTGGGAAAGCGC	55.0	50.0	58.0	58.0	219-250 bp	1.0	84.8
ndhH-rps15_p4	GAAAGGAGTCTGTGCACCAA	GAAAATTCTGGGAAAGCGCC	50.0	50.0	58.0	58.0	581-612 bp	1.0	82.0
ndhH-rps15_p5	GAAAGGAGTCTGTGCACCAA	GGAAAATTCTGGGAAAGCGC	50.0	50.0	58.0	58.0	582-613 bp	1.0	82.0