Hotspot

rpl32-trnL-UAG

SSC · 111168-111833 · 666 bp

Back to Cyclamen

Pi 0.0222
MarkerSeek score 63.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
666
variable_sites
25
pi
0.0222
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CTACCGGGCAGTCAAAAAGT TCATGCCCCTTTTTGACGAA 50.0 45.0 58.1 58.0 878-1148 bp 1.0 85.5
rpl32-trnL-UAG_p2 CTACCGGGCAGTCAAAAAGT TGCCCCTTTTTGACGAATCA 50.0 45.0 58.1 58.0 875-1145 bp 1.0 85.5
rpl32-trnL-UAG_p3 ATCTCTTTCTACCGGGCAGT TCATGCCCCTTTTTGACGAA 50.0 45.0 58.1 58.0 886-1156 bp 1.0 85.3
rpl32-trnL-UAG_p4 ATCTCTTTCTACCGGGCAGT TGCCCCTTTTTGACGAATCA 50.0 45.0 58.1 58.0 883-1153 bp 1.0 85.3
rpl32-trnL-UAG_p5 CTACCGGGCAGTCAAAAAGT ACTCAGATTGGAGAGCAGGT 50.0 50.0 58.1 58.1 1145-1415 bp 1.0 84.8