Hotspot

ndhF-rpl32

SSC · 110177-110987 · 811 bp

Back to Cyclamen

Pi 0.0242

MarkerSeek score 63.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 811
variable_sites: 34
pi: 0.0242
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACAAACGCCTTTTGACAAGC	ACTGCCCGGTAGAAAGAGAT	45.0	50.0	58.1	58.1	1477-1550 bp	1.0	81.7
ndhF-rpl32_p2	CAAACGCCTTTTGACAAGCA	ACTGCCCGGTAGAAAGAGAT	45.0	50.0	58.1	58.1	1476-1549 bp	1.0	81.7
ndhF-rpl32_p3	ACAAACGCCTTTTGACAAGC	ACTTTTTGACTGCCCGGTAG	45.0	50.0	58.1	58.1	1485 bp	0.25	48.5
ndhF-rpl32_p4	CAAACGCCTTTTGACAAGCA	ACTTTTTGACTGCCCGGTAG	45.0	50.0	58.1	58.1	1484 bp	0.25	48.5
ndhF-rpl32_p5	ACGAGCACATTCCAACCAAT	ACTTTTTGACTGCCCGGTAG	45.0	50.0	58.1	58.1	1421 bp	0.25	48.4