Hotspot

rpl36-rps8

LSC · 78058-78538 · 481 bp

Back to Cyclamen

Pi 0.0265

MarkerSeek score 66.30

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 481
variable_sites: 23
pi: 0.0265
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	CCTGCGGATTAGTCGACATT	CGACTAAAAGGAATAGGCGGA	50.0	47.6	57.8	57.6	577-583 bp	1.0	71.4
rpl36-rps8_p2	CCTGCGGATTAGTCGACATT	GAGAGGCTCGACTAAAAGGA	50.0	50.0	57.8	56.4	585-591 bp	1.0	70.0
rpl36-rps8_p3	CCTGCGGATTAGTCGACATT	AGGCGGAGAAATTTTGTGTT	50.0	40.0	57.8	56.1	563-569 bp	1.0	68.3
rpl36-rps8_p4	CCTGCGGATTAGTCGACATT	GAGAGGCTCGACTAAAAGGAA	50.0	47.6	57.8	57.1	585-591 bp	1.0	68.0
rpl36-rps8_p5	CCTGCGGATTAGTCGACATT	CGACTAAAAGGAATAGGCGG	50.0	50.0	57.8	56.0	577-583 bp	1.0	67.3