Hotspot

psbZ-trnG-GCC

LSC · 35653-35938 · 286 bp

Back to Cyclamen

Pi 0.0309

MarkerSeek score 61.70

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 286
variable_sites: 16
pi: 0.0309
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TGGAATAGCCGAGCCATTTC	AGTTCTTCTCTTTAGCGCGG	50.0	50.0	58.0	58.0	1153-1294 bp	1.0	85.7
psbZ-trnG-GCC_p2	TGGAATAGCCGAGCCATTTC	AATCCTGTCTCCGCAACATC	50.0	50.0	58.0	58.0	1080-1221 bp	1.0	85.6
psbZ-trnG-GCC_p3	TGGAATAGCCGAGCCATTTC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.0	58.0	1134-1275 bp	1.0	85.5
psbZ-trnG-GCC_p4	TGGAATAGCCGAGCCATTTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.0	58.0	1098-1239 bp	1.0	85.5
psbZ-trnG-GCC_p5	TGGAATAGCCGAGCCATTTC	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	1405-1547 bp	1.0	85.4