Hotspot

atpH-atpI

LSC · 13392-14074 · 683 bp

Back to Cyclamen

Pi 0.0233

MarkerSeek score 68.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 30
pi: 0.0233
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCTG	45.0	45.0	58.0	57.9	770-906 bp	1.0	81.8
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCCGCTG	50.0	45.0	57.9	57.9	826-962 bp	1.0	81.0
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCCGCTG	45.0	45.0	58.2	57.9	771-907 bp	1.0	80.6
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	TTTTGCAACTTTAGCCGCTG	55.0	45.0	58.2	57.9	830-966 bp	1.0	80.3
atpH-atpI_p5	TCCAGCAGCAATAACGGAAG	TTTTGCAACTTTAGCCGCTG	50.0	45.0	58.3	57.9	780-916 bp	1.0	79.9