Hotspot

trnS-GCU-trnS-CGA

LSC · 7901-8617 · 717 bp

Back to Cyclamen

Pi 0.0397

MarkerSeek score 75.70

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 717
variable_sites: 51
pi: 0.0397
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	CTTTCGTCCACTCAGCCATC	ACCACTAAACTATACCCGCT	55.0	45.0	58.6	55.6	749-790 bp	1.0	63.5
trnS-GCU-trnS-CGA_p2	TTTCGTCCACTCAGCCATCT	ACCACTAAACTATACCCGCT	50.0	45.0	59.0	55.6	748-789 bp	1.0	60.5
trnS-GCU-trnS-CGA_p3	TCCACTCAGCCATCTCTCC	ACCACTAAACTATACCCGCT	57.9	45.0	58.1	55.6	743-784 bp	1.0	59.9
trnS-GCU-trnS-CGA_p4	ATTAGCAATCCGCCGCTTT	ACCACTAAACTATACCCGCT	47.4	45.0	58.2	55.6	764-805 bp	1.0	58.8
trnS-GCU-trnS-CGA_p5	ACTCGTACAACGGATTAGCA	ACCACTAAACTATACCCGCT	45.0	45.0	56.7	55.6	777-818 bp	1.0	57.9