Hotspot

trnK-UUU-rps16

LSC · 4293-5110 · 818 bp

Back to Cyclamen

Pi 0.0281

MarkerSeek score 65.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 43
pi: 0.0281
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CACTTCAGGATCAGTCGTGG	CTATCCCACTGAGCCGTCTA	55.0	55.0	58.0	58.0	1186-1203 bp	1.0	84.3
trnK-UUU-rps16_p2	CCACTTCAGGATCAGTCGTG	CTATCCCACTGAGCCGTCTA	55.0	55.0	58.0	58.0	1187-1204 bp	1.0	84.3
trnK-UUU-rps16_p3	CACTTCAGGATCAGTCGTGG	CTGAGCCGTCTATCGAATCG	55.0	55.0	58.0	58.0	1178-1195 bp	1.0	84.2
trnK-UUU-rps16_p4	CCACTTCAGGATCAGTCGTG	CTGAGCCGTCTATCGAATCG	55.0	55.0	58.0	58.0	1179-1196 bp	1.0	84.2
trnK-UUU-rps16_p5	CACTTCAGGATCAGTCGTGG	GCCGTACGAGGAGAAAACTT	55.0	50.0	58.0	57.9	1242-1260 bp	1.0	83.9