Hotspot

rps19

LSC · 86205-86483 · 279 bp

Back to Cycas

Pi 0.00287
MarkerSeek score 24.90
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
LSC
length_bp
279
variable_sites
6
pi
0.00287
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
24.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 GCAAACCAATGGGAACGATG CTTCAATCCTTCGTCGACGT 50.0 50.0 58.0 58.0 878-907 bp 1.0 84.2
rps19_p2 GCAAACCAATGGGAACGATG TGTTCAGCAACGATTGGACA 50.0 45.0 58.0 58.0 1119-1148 bp 1.0 83.3
rps19_p3 GCAAACCAATGGGAACGATG GGATGTAGAGGATCGGGACA 50.0 55.0 58.0 58.0 1480-1509 bp 1.0 82.9
rps19_p4 GCAAACCAATGGGAACGATG CGGGACATGAGGAACGAAAT 50.0 50.0 58.0 58.0 1467-1496 bp 1.0 82.8
rps19_p5 TTCCCCGACCCCTATTTTCT GGATGTAGAGGATCGGGACA 50.0 55.0 58.0 58.0 1684-1712 bp 0.981132 81.8