Hotspot

ycf1-chlN

SSC · 133801-134114 · 314 bp

Back to Cycas

Pi 0.00638
MarkerSeek score 40.30
Primer available yes
Label ycf1-chlN

Feature Metadata

label_name
ycf1-chlN
feature_type
igs
region
SSC
length_bp
314
variable_sites
9
pi
0.00638
unique_haplotype_count
24
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-chlN_p1 GGACTAATGGGCAATGTGGT ATTTAGAAGACTTGGGCCGG 50.0 50.0 57.9 57.6 450-483 bp 1.0 81.0
ycf1-chlN_p2 GGACTAATGGGCAATGTGGT TTTAGAAGACTTGGGCCGGA 50.0 50.0 57.9 58.6 449-482 bp 1.0 79.2
ycf1-chlN_p3 AGGACTAATGGGCAATGTGG ATTTAGAAGACTTGGGCCGG 50.0 50.0 57.6 57.6 451-484 bp 1.0 78.6
ycf1-chlN_p4 AGGACTAATGGGCAATGTGG TTTAGAAGACTTGGGCCGGA 50.0 50.0 57.6 58.6 450-483 bp 1.0 76.8
ycf1-chlN_p5 GGACTAATGGGCAATGTGGT CTTTGCGGTGTAACGACAAT 50.0 45.0 57.9 57.0 468-501 bp 1.0 76.6