Hotspot

rpl32-trnP-GGG

SSC · 117261-118382 · 1122 bp

Back to Cycas

Pi 0.00484
MarkerSeek score 52.10
Primer available yes
Label rpl32-trnP-GGG

Feature Metadata

label_name
rpl32-trnP-GGG
feature_type
igs
region
SSC
length_bp
1122
variable_sites
58
pi
0.00484
unique_haplotype_count
42
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnP-GGG_p1 CATCATGGCAGTTCCGAAGA TCCCGAATAAAGCCAACGAG 50.0 50.0 58.0 58.0 1673-2017 bp 1.0 86.8
rpl32-trnP-GGG_p2 TCATCATGGCAGTTCCGAAG TCCCGAATAAAGCCAACGAG 50.0 50.0 58.0 58.0 1674-2018 bp 1.0 86.8
rpl32-trnP-GGG_p3 CAGATCAGACCGCGGTAAAA GGCGGCTTGGTAAAGACATA 50.0 50.0 58.0 58.0 1170-1514 bp 0.849057 80.7
rpl32-trnP-GGG_p4 CAGATCAGACCGCGGTAAAA TCCCGAATAAAGCCAACGAG 50.0 50.0 58.0 58.0 1599-1943 bp 0.849057 80.6
rpl32-trnP-GGG_p5 CAGATCAGACCGCGGTAAAA TTCTAAGAATGCCGCCACTC 50.0 50.0 58.0 58.0 1253-1597 bp 0.849057 80.6