Hotspot

ndhF-rpl32

SSC · 115898-117050 · 1153 bp

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Pi 0.00638
MarkerSeek score 59.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1153
variable_sites
64
pi
0.00638
unique_haplotype_count
49
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAAGAAAGGCACGATCCA TCTGGATTTAGAAGTGCGCT 50.0 45.0 58.2 56.9 1212-1263 bp 1.0 77.2
ndhF-rpl32_p2 TGGAAAGAAAGGCACGATCC TCTGGATTTAGAAGTGCGCT 50.0 45.0 58.2 56.9 1213-1264 bp 1.0 77.2
ndhF-rpl32_p3 GGAAAGAAAGGCACGATCCA GCTTCTTCGGAACTGCCAT 50.0 52.6 58.2 58.1 1195-1246 bp 1.0 76.6
ndhF-rpl32_p4 TGGAAAGAAAGGCACGATCC GCTTCTTCGGAACTGCCAT 50.0 52.6 58.2 58.1 1196-1247 bp 1.0 76.6
ndhF-rpl32_p5 AGAAAGGCACGATCCAAACA TCTGGATTTAGAAGTGCGCT 45.0 45.0 57.7 56.9 1208-1259 bp 1.0 76.5