Hotspot

rps19-rpl2

LSC · 86484-86547 · 64 bp

Back to Cycas

Pi 0.0471
MarkerSeek score 70.30
Primer available yes
Label rps19-rpl2

Feature Metadata

label_name
rps19-rpl2
feature_type
igs
region
LSC
length_bp
64
variable_sites
16
pi
0.0471
unique_haplotype_count
43
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl2_p1 AGGTGCAAATTCCCCCAATT CTTCAATCCTTCGTCGACGT 45.0 50.0 58.0 58.0 315-344 bp 1.0 88.7
rps19-rpl2_p2 AGGTGCAAATTCCCCCAATT GGATGTAGAGGATCGGGACA 45.0 55.0 58.0 58.0 917-946 bp 1.0 83.4
rps19-rpl2_p3 AGGTGCAAATTCCCCCAATT CGGGACATGAGGAACGAAAT 45.0 50.0 58.0 58.0 904-933 bp 1.0 83.2
rps19-rpl2_p4 GTAGGTGCAAATTCCCCCAA GGATGTAGAGGATCGGGACA 50.0 55.0 58.1 58.0 919-948 bp 1.0 83.1
rps19-rpl2_p5 TACTTCGGCCCTACTGACAA GGATGTAGAGGATCGGGACA 50.0 55.0 58.1 58.0 1254-1283 bp 1.0 82.5