Hotspot

petA-psbJ

LSC · 65200-66303 · 1104 bp

Back to Cycas

Pi 0.00421
MarkerSeek score 57.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1104
variable_sites
37
pi
0.00421
unique_haplotype_count
28
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCATCTGTCATTTTGGCAC ACTGGTACCCTTGTGATTGG 50.0 50.0 58.0 57.1 1221-1264 bp 1.0 78.2
petA-psbJ_p2 GGCATCTGTCATTTTGGCAC CTGGTACCCTTGTGATTGGT 50.0 50.0 58.0 57.1 1220-1263 bp 1.0 78.2
petA-psbJ_p3 GGCATCTGTCATTTTGGCAC AACTGGTACCCTTGTGATTGG 50.0 47.6 58.0 57.8 1222-1265 bp 1.0 76.0
petA-psbJ_p4 GGCATCTGTCATTTTGGCAC CTGGTACCCTTGTGATTGGTT 50.0 47.6 58.0 57.8 1220-1263 bp 1.0 76.0
petA-psbJ_p5 CTGGCATCTGTCATTTTGGC CTGGTACCCTTGTGATTGGT 50.0 50.0 57.7 57.1 1222-1265 bp 1.0 75.9