Hotspot

atpB-rbcL

LSC · 57068-57764 · 697 bp

Back to Cycas

Pi 0.00438
MarkerSeek score 53.10
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
697
variable_sites
21
pi
0.00438
unique_haplotype_count
34
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CCCAAGAGCAAGAGGATTGG TCCAACACTTGCCTTAGTCTC 55.0 47.6 58.2 57.6 745-761 bp 1.0 72.3
atpB-rbcL_p2 CCCCAAGAGCAAGAGGATTG TCCAACACTTGCCTTAGTCTC 55.0 47.6 58.2 57.6 746-762 bp 1.0 72.3
atpB-rbcL_p3 CCCAAGAGCAAGAGGATTGG CCAACACTTGCCTTAGTCTCT 55.0 47.6 58.2 57.6 744-760 bp 1.0 72.2
atpB-rbcL_p4 CCCCAAGAGCAAGAGGATTG CCAACACTTGCCTTAGTCTCT 55.0 47.6 58.2 57.6 745-761 bp 1.0 72.2
atpB-rbcL_p5 CCAGTACTGGGCCAATGATT TCCAACACTTGCCTTAGTCTC 50.0 47.6 57.6 57.6 806-822 bp 1.0 70.3