Hotspot

atpE-atpB

LSC · 55581-55588 · 8 bp

Back to Cycas

Pi 0.0142
MarkerSeek score 53.70
Primer available yes
Label atpE-atpB

Feature Metadata

label_name
atpE-atpB
feature_type
igs
region
LSC
length_bp
8
variable_sites
3
pi
0.0142
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpE-atpB_p1 CGCCAATTTGACCGCTATTG CCGCGAAGGCTATGAACTTA 50.0 50.0 58.2 57.8 140-141 bp 1.0 78.6
atpE-atpB_p2 CGCCAATTTGACCGCTATTG CTTCCCGAACAGGCCTTTTA 50.0 50.0 58.2 57.8 186-187 bp 1.0 78.3
atpE-atpB_p3 CGCCAATTTGACCGCTATTG ATTGATGAAGCTACCGCGAA 50.0 45.0 58.2 57.7 153-154 bp 1.0 77.7
atpE-atpB_p4 ATACGCCAATTTGACCGCTA CTTCCCGAACAGGCCTTTTA 45.0 50.0 57.7 57.8 189-190 bp 1.0 77.2
atpE-atpB_p5 TACGCCAATTTGACCGCTAT CTTCCCGAACAGGCCTTTTA 45.0 50.0 57.7 57.8 188-189 bp 1.0 77.2