Hotspot

ycf3-trnS-GGA

LSC · 44659-45423 · 765 bp

Back to Cycas

Pi 0.00477
MarkerSeek score 58.90
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
765
variable_sites
34
pi
0.00477
unique_haplotype_count
28
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 AATGCCTCTTTTTCCCCTGG GTGCGGAAAGAGAGGGATTC 50.0 55.0 58.1 58.3 939-997 bp 1.0 81.7
ycf3-trnS-GGA_p2 CTCTTTTTCCCCTGGAGCTG GTGCGGAAAGAGAGGGATTC 55.0 55.0 58.2 58.3 934-992 bp 1.0 80.8
ycf3-trnS-GGA_p3 AATGCCTCTTTTTCCCCTGG GGATTCGAACCCTCGGTAAA 50.0 50.0 58.1 57.3 925-983 bp 1.0 79.0
ycf3-trnS-GGA_p4 GGAGCTGTGGGGATTATTCG GTGCGGAAAGAGAGGGATTC 55.0 55.0 58.1 58.3 921-979 bp 0.924528 78.1
ycf3-trnS-GGA_p5 GGAGCTGTGGGGATTATTCG GGATTCGAACCCTCGGTAAA 55.0 50.0 58.1 57.3 907-965 bp 0.924528 75.4