Hotspot

psbM-trnD-GUC

LSC · 30189-31300 · 1112 bp

Back to Cycas

Pi 0.00326
MarkerSeek score 51.40
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1112
variable_sites
29
pi
0.00326
unique_haplotype_count
24
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 CCTGGAGTATGAGATTGGACG ATTGGTCAGAGTACCGTCCT 52.4 50.0 57.6 57.8 1253-1270 bp 1.0 69.7
psbM-trnD-GUC_p2 CCTGGAGTATGAGATTGGACG TCAGAGTACCGTCCTGTCAA 52.4 50.0 57.6 57.7 1248-1265 bp 1.0 69.3
psbM-trnD-GUC_p3 CCTGGAGTATGAGATTGGACG GTCAGAGTACCGTCCTGTCA 52.4 55.0 57.6 58.5 1249-1266 bp 1.0 67.8
psbM-trnD-GUC_p4 TCCCTGGAGTATGAGATTGGA ATTGGTCAGAGTACCGTCCT 47.6 50.0 57.3 57.8 1255-1272 bp 1.0 67.1
psbM-trnD-GUC_p5 CCTGGAGTATGAGATTGGACG AGAGTACCGTCCTGTCAAGA 52.4 50.0 57.6 57.4 1246-1263 bp 1.0 66.9