Hotspot

atpH-atpI

LSC · 15147-15758 · 612 bp

Back to Cycas

Pi 0.00448
MarkerSeek score 56.80
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
612
variable_sites
21
pi
0.00448
unique_haplotype_count
30
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 TAACGGAAGCAGCAGGAATC CTTTGCAACTTTAGCCGCAG 50.0 50.0 58.0 58.3 698-718 bp 1.0 81.4
atpH-atpI_p2 GCAGCAGGAATCAAGGGATT CTTTGCAACTTTAGCCGCAG 50.0 50.0 58.2 58.3 690-710 bp 1.0 79.8
atpH-atpI_p3 CAATAACGGAAGCAGCAGGA CTTTGCAACTTTAGCCGCAG 50.0 50.0 58.3 58.3 701-721 bp 1.0 79.5
atpH-atpI_p4 TCCAGCAGCAATAACGGAAG CTTTGCAACTTTAGCCGCAG 50.0 50.0 58.3 58.3 709-729 bp 1.0 79.4
atpH-atpI_p5 CAATACCCTCTACGGCTTGG CTTTGCAACTTTAGCCGCAG 55.0 50.0 57.8 58.3 785-805 bp 1.0 79.2