Hotspot

psbK-psbI

LSC · 8493-9249 · 757 bp

Back to Cycas

Pi 0.00467
MarkerSeek score 46.90
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
757
variable_sites
30
pi
0.00467
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG CGTCCAGGATCGTTCGATAG 50.0 55.0 57.9 57.7 876-898 bp 1.0 79.9
psbK-psbI_p2 GCCAAATTACCCGAGGCTTA CGTCCAGGATCGTTCGATAG 50.0 55.0 58.0 57.7 963-985 bp 1.0 79.8
psbK-psbI_p3 GTTTGGCAAGCTGCTGTAAG ACGTCCAGGATCGTTCGATA 50.0 50.0 57.9 58.3 877-899 bp 1.0 79.8
psbK-psbI_p4 GCCAAATTACCCGAGGCTTA ACGTCCAGGATCGTTCGATA 50.0 50.0 58.0 58.3 964-986 bp 1.0 79.7
psbK-psbI_p5 GCCAAATTACCCGAGGCTTA TACGTCCAGGATCGTTCGAT 50.0 50.0 58.0 58.3 965-987 bp 1.0 79.7