Hotspot

trnR-UCU

LSC · 9545-9616 · 72 bp

Back to Cyathula

Pi 0.0139

MarkerSeek score 60.50

Primer available yes

Label trnR-UCU

Feature Metadata

label_name: trnR-UCU
feature_type: tRNA
region: LSC
length_bp: 72
variable_sites: 1
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU_p1	TCTCACATCACATACAATCCGA	TCAAGAACAGAAGGAACGCT	40.9	45.0	57.0	57.1	247 bp	1.0	49.0
trnR-UCU_p2	TCTCACATCACATACAATCCGA	TCAAGAACAGAAGGAACGCTT	40.9	42.9	57.0	57.8	247 bp	1.0	46.8
trnR-UCU_p3	TCTCACATCACATACAATCCGA	TTCAAGAACAGAAGGAACGCT	40.9	42.9	57.0	57.8	248 bp	1.0	46.8
trnR-UCU_p4	TCTCACATCACATACAATCCGA	GGAACGCTTTCTACTTCAGGA	40.9	47.6	57.0	57.7	235 bp	1.0	45.8
trnR-UCU_p5	TCTCACATCACATACAATCCGA	AGGAACGCTTTCTACTTCAGG	40.9	47.6	57.0	57.7	236 bp	1.0	45.8