Hotspot

rps15

SSC · 121793-122065 · 273 bp

Back to Cyathula

Pi 0.00366

MarkerSeek score 54.60

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 1
pi: 0.00366
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	ACAGTCATAGGTTTTTCCCCG	ACCAAATCAAAATAGCTGGCA	47.6	38.1	57.9	56.4	483 bp	1.0	50.7
rps15_p2	CAGTCATAGGTTTTTCCCCGA	ACCAAATCAAAATAGCTGGCA	47.6	38.1	57.7	56.4	482 bp	1.0	48.5
rps15_p3	ACAGTCATAGGTTTTTCCCCG	ACCAAATCAAAATAGCTGGCAT	47.6	36.4	57.9	56.9	483 bp	1.0	46.8
rps15_p4	CAGTCATAGGTTTTTCCCCGA	ACCAAATCAAAATAGCTGGCAT	47.6	36.4	57.7	56.9	482 bp	1.0	44.6
rps15_p5	AGTCATAGGTTTTTCCCCGA	ACCAAATCAAAATAGCTGGCA	45.0	38.1	56.1	56.4	481 bp	1.0	44.4