Hotspot

rpoC1

LSC · 20167-22967 · 2801 bp

Back to Cyathula

Pi 0.000357

MarkerSeek score 56.20

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2801
variable_sites: 1
pi: 0.000357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	AAAGACTCGAATTCTGCCGA	ACGATCTTTGGCTCTGGAAC	45.0	50.0	57.3	57.9	2965-2966 bp	1.0	73.3
rpoC1_p2	AAGACTCGAATTCTGCCGAT	ACGATCTTTGGCTCTGGAAC	45.0	50.0	57.0	57.9	2964-2965 bp	1.0	71.5
rpoC1_p3	AAAGACTCGAATTCTGCCGA	CGATCTTTGGCTCTGGAACT	45.0	50.0	57.3	57.6	2964-2965 bp	1.0	70.9
rpoC1_p4	GAAAGACTCGAATTCTGCCG	ACGATCTTTGGCTCTGGAAC	50.0	50.0	56.9	57.9	2966-2967 bp	1.0	70.6
rpoC1_p5	AAAGACTCGAATTCTGCCGA	GCTCGTTCGAGAACTACGAT	45.0	50.0	57.3	57.5	2980-2981 bp	1.0	70.4