Hotspot

rpl16

LSC · 80320-81723 · 1404 bp

Back to Cyathula

Pi 0.000712

MarkerSeek score 59.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1404
variable_sites: 1
pi: 0.000712
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACTGTTTGGCCAATTGTCCA	AGAATGCCAACTTTGTTCATTCT	45.0	34.8	58.2	57.2	1581 bp	1.0	48.0
rpl16_p2	ACTGTTTGGCCAATTGTCCA	AAGAATGCCAACTTTGTTCA	45.0	35.0	58.2	53.9	1582 bp	1.0	45.4
rpl16_p3	ACTGTTTGGCCAATTGTCCA	TGCCAACTTTGTTCATTCTT	45.0	35.0	58.2	53.9	1577 bp	1.0	45.4
rpl16_p4	ACTGTTTGGCCAATTGTCCA	AAGAATGCCAACTTTGTTCATTCT	45.0	33.3	58.2	57.8	1582 bp	1.0	45.0
rpl16_p5	ACTGTTTGGCCAATTGTCCA	AGAATGCCAACTTTGTTCATTCTT	45.0	33.3	58.2	57.8	1581 bp	1.0	45.0