Hotspot

petB

LSC · 74026-75414 · 1389 bp

Back to Cyathula

Pi 0
MarkerSeek score 40.00
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
1389
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 CCCTTTTGGTAGTTCGATCGT ACCGTTGTTCTTCTCCCAAG 47.6 50.0 58.0 57.7 1547 bp 1.0 69.7
petB_p2 CCCTTTTGGTAGTTCGATCGT CCGTTGTTCTTCTCCCAAGT 47.6 50.0 58.0 57.7 1546 bp 1.0 69.7
petB_p3 TTGGTAGTTCGATCGTGGAA ACCGTTGTTCTTCTCCCAAG 45.0 50.0 56.6 57.7 1542 bp 1.0 66.7
petB_p4 TTGGTAGTTCGATCGTGGAA CCGTTGTTCTTCTCCCAAGT 45.0 50.0 56.6 57.7 1541 bp 1.0 66.7
petB_p5 TTTGGTAGTTCGATCGTGGA CCGTTGTTCTTCTCCCAAGT 45.0 50.0 56.6 57.7 1542 bp 1.0 66.7