Hotspot

ndhF

SSC · 108885-111122 · 2238 bp

Back to Cyathula

Pi 0.000449

MarkerSeek score 52.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 1
pi: 0.000449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AATTTGCTGGGGAATCGACA	TCTTTCAAAAGAGGCAAGAGC	45.0	42.9	57.8	56.7	2430 bp	0.5	40.1
ndhF_p2	TTTGCTGGGGAATCGACATT	TCTTTCAAAAGAGGCAAGAGC	45.0	42.9	57.8	56.7	2428 bp	0.5	40.1
ndhF_p3	GCTGGGGAATCGACATTGAA	TCTTTCAAAAGAGGCAAGAGC	50.0	42.9	58.2	56.7	2425 bp	0.5	39.8
ndhF_p4	ATTTGCTGGGGAATCGACAT	TCTTTCAAAAGAGGCAAGAGC	45.0	42.9	57.6	56.7	2429 bp	0.5	38.3
ndhF_p5	TCAATTTGCTGGGGAATCGA	TCTTTCAAAAGAGGCAAGAGC	45.0	42.9	57.5	56.7	2432 bp	0.5	37.8