Hotspot

psbZ-trnG-GCC

LSC · 35775-36082 · 308 bp

Back to Cyathula

Pi 0.00325

MarkerSeek score 54.50

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 308
variable_sites: 1
pi: 0.00325
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TGTATTTGCTTCTCCCGACG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.0	57.8	470 bp	1.0	78.8
psbZ-trnG-GCC_p2	CGACGGTTGGTCAAGTAACA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.1	57.8	455 bp	1.0	77.7
psbZ-trnG-GCC_p3	GTATTTGCTTCTCCCGACGG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.7	57.8	469 bp	1.0	73.1
psbZ-trnG-GCC_p4	CCGACGGTTGGTCAAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.9	57.8	456 bp	1.0	72.0
psbZ-trnG-GCC_p5	TGTATTTGCTTCTCCCGACG	CGGATAGCGGGAATCGAAC	50.0	57.9	58.0	58.2	492 bp	1.0	70.6