Hotspot

psbM-trnD-GUC

LSC · 29232-30227 · 996 bp

Back to Cyathula

Pi 0
MarkerSeek score 26.80
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
996
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
26.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TCATTTTGGCTGACCGTTTT GTTCGAGCCCCGTCAGTC 40.0 66.7 56.4 60.1 1118 bp 1.0 40.0
psbM-trnD-GUC_p2 AATCATTTTGGCTGACCGTT GTTCGAGCCCCGTCAGTC 40.0 66.7 56.2 60.1 1120 bp 1.0 40.0
psbM-trnD-GUC_p3 ATCATTTTGGCTGACCGTTT GTTCGAGCCCCGTCAGTC 40.0 66.7 56.2 60.1 1119 bp 1.0 40.0
psbM-trnD-GUC_p4 TCATTTTGGCTGACCGTTTTT GTTCGAGCCCCGTCAGTC 38.1 66.7 57.2 60.1 1118 bp 1.0 40.0
psbM-trnD-GUC_p5 TTTGGCTGACCGTTTTTACA GTTCGAGCCCCGTCAGTC 40.0 66.7 56.1 60.1 1114 bp 1.0 40.0