Hotspot

ycf2

Genome · 29978-36109 · 6132 bp

Back to Cyathodium

Pi 0.000326

MarkerSeek score 52.70

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6132
variable_sites: 2
pi: 0.000326
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TCTAGGATGCTTAGCCGGAA	ACCTGATGAAAATCTTGGATCGA	50.0	39.1	57.9	57.9	282 bp	1.0	54.8
ycf2_p2	TTCTAGGATGCTTAGCCGGA	ACCTGATGAAAATCTTGGATCGA	50.0	39.1	57.9	57.9	283 bp	1.0	54.8
ycf2_p3	TCTAGGATGCTTAGCCGGAA	CACCTGATGAAAATCTTGGATCG	50.0	43.5	57.9	57.8	283 bp	1.0	54.3
ycf2_p4	TTCTAGGATGCTTAGCCGGA	CACCTGATGAAAATCTTGGATCG	50.0	43.5	57.9	57.8	284 bp	1.0	54.3
ycf2_p5	CTAGGATGCTTAGCCGGAAC	ACCTGATGAAAATCTTGGATCGA	55.0	39.1	57.8	57.9	281 bp	1.0	54.2