Hotspot

rps7

Genome · 966-1433 · 468 bp

Back to Cyathodium

Pi 0.256

MarkerSeek score 76.00

Primer available yes

Label rps7

Feature Metadata

label_name: rps7
feature_type: gene
region: Genome
length_bp: 468
variable_sites: 120
pi: 0.256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7_p1	TACGCGTTAGCTACACAACC	CGTAAGGGGTTTTGGAGGAG	50.0	55.0	58.0	57.9	1094 bp	0.5	59.0
rps7_p2	TACGCGTTAGCTACACAACC	GACGTAAGGGGTTTTGGAGG	50.0	55.0	58.0	58.2	1096 bp	0.5	58.4
rps7_p3	TCAACAAGGGCGTTCTAGTG	CGTAAGGGGTTTTGGAGGAG	50.0	55.0	57.8	57.9	1305 bp	0.5	57.8
rps7_p4	GTCAACAAGGGCGTTCTAGT	CGTAAGGGGTTTTGGAGGAG	50.0	55.0	57.8	57.9	1306 bp	0.5	57.8
rps7_p5	TCAACAAGGGCGTTCTAGTG	GACGTAAGGGGTTTTGGAGG	50.0	55.0	57.8	58.2	1307 bp	0.5	57.2