Hotspot

rps12

Genome · 81-908 · 828 bp

Back to Cyathodium

Pi 0.302

MarkerSeek score 82.00

Primer available yes

Label rps12

Feature Metadata

label_name: rps12
feature_type: gene
region: Genome
length_bp: 828
variable_sites: 222
pi: 0.302
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12_p1	CGGGAAGCCGTATGAAATGA	ACTTTCTTCCCTCTTCCCGA	50.0	50.0	58.1	58.0	1388 bp	0.5	59.4
rps12_p2	CGGGAAGCCGTATGAAATGA	TTTTCCTTGTGCCGAGACAA	50.0	45.0	58.1	58.0	1256 bp	0.5	59.2
rps12_p3	AGTCCGGTTTCGTAGAGTGA	ACTTTCTTCCCTCTTCCCGA	50.0	50.0	58.1	58.0	1360 bp	0.5	59.0
rps12_p4	AGTCCGGTTTCGTAGAGTGA	TTTTCCTTGTGCCGAGACAA	50.0	45.0	58.1	58.0	1228 bp	0.5	58.8
rps12_p5	CGGGAAGCCGTATGAAATGA	CGTAAGGGGTTTTGGAGGAG	50.0	55.0	58.1	57.9	1600 bp	0.5	58.7