Hotspot

chlL

Genome · 108093-108962 · 870 bp

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Pi 0

MarkerSeek score 26.50

Primer available yes

Label chlL

Feature Metadata

label_name: chlL
feature_type: gene
region: Genome
length_bp: 870
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL_p1	TTCTGTTGTGCACGTACCAA	GAGTCCCCCTCTCCGATAAA	45.0	55.0	58.0	57.9	1549-1659 bp	1.0	83.2
chlL_p2	TTTCTGTTGTGCACGTACCA	GAGTCCCCCTCTCCGATAAA	45.0	55.0	58.0	57.9	1550-1660 bp	1.0	83.2
chlL_p3	TTCTGTTGTGCACGTACCAA	GCTACAGAGACTTCTTCCGC	45.0	55.0	58.0	58.1	1650-1760 bp	1.0	83.2
chlL_p4	TTTCTGTTGTGCACGTACCA	GCTACAGAGACTTCTTCCGC	45.0	55.0	58.0	58.1	1651-1761 bp	1.0	83.2
chlL_p5	TGTGCACGTACCAATCCAAA	GAGTCCCCCTCTCCGATAAA	45.0	55.0	58.0	57.9	1543-1653 bp	1.0	83.2