Hotspot

atpI

Genome · 16913-17659 · 747 bp

Back to Cyathodium

Pi 0.00134
MarkerSeek score 59.50
Primer available yes
Label atpI

Feature Metadata

label_name
atpI
feature_type
gene
region
Genome
length_bp
747
variable_sites
1
pi
0.00134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI_p1 TGACAAACCGTTTTATGTGTTTCT AGATTCAAGGTGGAATGAAAACA 33.3 34.8 57.8 56.6 926 bp 1.0 40.1
atpI_p2 TGACAAACCGTTTTATGTGT AGATTCAAGGTGGAATGAAAACA 35.0 34.8 53.7 56.6 926 bp 1.0 40.1
atpI_p3 TGACAAACCGTTTTATGTGTT AGATTCAAGGTGGAATGAAAACA 33.3 34.8 54.5 56.6 926 bp 1.0 40.1
atpI_p4 TGACAAACCGTTTTATGTGTTTC AGATTCAAGGTGGAATGAAAACA 34.8 34.8 56.5 56.6 926 bp 1.0 40.1
atpI_p5 TGACAAACCGTTTTATGTGTTTCT AGATTCAAGGTGGAATGAAAACAA 33.3 33.3 57.8 57.3 926 bp 1.0 40.1