Hotspot

psaM-trnS-GCU

Genome · 22633-23130 · 498 bp

Back to Cyathodium

Pi 0.0714

MarkerSeek score 67.20

Primer available yes

Label psaM-trnS-GCU

Feature Metadata

label_name: psaM-trnS-GCU
feature_type: igs
region: Genome
length_bp: 498
variable_sites: 1
pi: 0.0714
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.03
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaM-trnS-GCU_p1	AGCTTTAAGACTAGGGAAGGA	TACGGAGAGAGAGGGATTCG	42.9	55.0	55.4	57.7	138-622 bp	1.0	53.3
psaM-trnS-GCU_p2	AGCTTTAAGACTAGGGAAGGA	CGGAGAGAGAGGGATTCGAA	42.9	55.0	55.4	58.3	136-620 bp	1.0	52.9
psaM-trnS-GCU_p3	AGCTTTAAGACTAGGGAAGGAA	TACGGAGAGAGAGGGATTCG	40.9	55.0	56.2	57.7	138-622 bp	1.0	51.6
psaM-trnS-GCU_p4	AGCTTTAAGACTAGGGAAGGAA	CGGAGAGAGAGGGATTCGAA	40.9	55.0	56.2	58.3	136-620 bp	1.0	51.1
psaM-trnS-GCU_p5	AGCTTTAAGACTAGGGAAGGA	GATTCGAACCCTCGGTACAA	42.9	50.0	55.4	57.3	124-608 bp	1.0	50.1