Hotspot

rps7-ndhB

Genome · 1434-1602 · 169 bp

Back to Cyathodium

Pi 0.421

MarkerSeek score 67.30

Primer available yes

Label rps7-ndhB

Feature Metadata

label_name: rps7-ndhB
feature_type: igs
region: Genome
length_bp: 169
variable_sites: 40
pi: 0.421
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.56
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-ndhB_p1	TCGGGAAGAGGGAAGAAAGT	AACGAATCGCACTCCTTCAT	50.0	45.0	58.0	57.6	972 bp	0.5	56.7
rps7-ndhB_p2	TCGGGAAGAGGGAAGAAAGT	CCGTTTCTCCACCGGATAAA	50.0	50.0	58.0	57.6	811 bp	0.5	56.7
rps7-ndhB_p3	TTGTCTCGGCACAAGGAAAA	AACGAATCGCACTCCTTCAT	45.0	45.0	58.0	57.6	1104 bp	0.5	56.5
rps7-ndhB_p4	TTGTCTCGGCACAAGGAAAA	CCGTTTCTCCACCGGATAAA	45.0	50.0	58.0	57.6	943 bp	0.5	56.5
rps7-ndhB_p5	TCGGGAAGAGGGAAGAAAGT	TCCGTTTCTCCACCGGATAA	50.0	50.0	58.0	58.4	812 bp	0.5	56.3