Hotspot

clpP

LSC · 78827-82243 · 3417 bp

Back to Cyanotis

Pi 0.0539

MarkerSeek score 69.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 3417
variable_sites: 146
pi: 0.0539
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CAAGTATCCAGGCTCCGTTC	GCTGAGATATTTATTCCTAATATTTGG	55.0	29.6	58.1	54.7	805-1405 bp	1.0	51.4
clpP_p2	TATCCAGGCTCCGTTCAGAA	GCTGAGATATTTATTCCTAATATTTGG	50.0	29.6	58.1	54.7	801-1401 bp	1.0	51.4
clpP_p3	AGGGTTCACTTGGACTGAGA	GCTGAGATATTTATTCCTAATATTTGG	50.0	29.6	57.9	54.7	835-1435 bp	1.0	51.2
clpP_p4	TTGGGTTGACGTATAGTGCG	GCTGAGATATTTATTCCTAATATTTGG	50.0	29.6	58.0	54.7	1029-1620 bp	1.0	50.2
clpP_p5	AGTGACATTCGATTCGGAGC	GCTGAGATATTTATTCCTAATATTTGG	50.0	29.6	58.1	54.7	1519 bp	0.5	20.0