Hotspot

ndhF-rpl32

SSC · 126910-128224 · 1315 bp

Back to Cyanotis

Pi 0.195

MarkerSeek score 85.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1315
variable_sites: 247
pi: 0.195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGTATCATCCATGCATATTGA	TTGACAAAGAAGTACGTTTTT	38.1	28.6	53.2	52.2	1384-1551 bp	1.0	50.7
ndhF-rpl32_p2	GGTATCATCCATGCATATTGA	TTTGACAAAGAAGTACGTTTT	38.1	28.6	53.2	52.2	1385-1552 bp	1.0	50.7
ndhF-rpl32_p3	GGTATCATCCATGCATATTGA	TTTTGACAAAGAAGTACGTTT	38.1	28.6	53.2	52.2	1386-1553 bp	1.0	50.7
ndhF-rpl32_p4	GGTATCATCCATGCATATTGA	TTTTTGACAAAGAAGTACGTT	38.1	28.6	53.2	52.2	1387-1554 bp	1.0	50.7
ndhF-rpl32_p5	GGTATCATCCATGCATATTGA	TTTGACAAAGAAGTACGTTTTT	38.1	27.3	53.2	53.1	1385-1552 bp	1.0	50.7