Hotspot

rpl16-rps3

LSC · 93501-94980 · 1480 bp

Back to Cyanotis

Pi 0.0608

MarkerSeek score 78.00

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1480
variable_sites: 82
pi: 0.0608
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GCGGGTCTTACTGTAACAGG	AACCTTACGGCCAACCAAAT	55.0	45.0	58.0	58.0	1941-2024 bp	1.0	87.7
rpl16-rps3_p2	CGTGCATATCGTGTCATTGC	AACCTTACGGCCAACCAAAT	50.0	45.0	58.0	58.0	1881-1964 bp	1.0	87.7
rpl16-rps3_p3	CTTCGCCCTGCTTCAATTTG	AACCTTACGGCCAACCAAAT	50.0	45.0	58.0	58.0	1860-1943 bp	1.0	87.7
rpl16-rps3_p4	GCGGGTCTTACTGTAACAGG	GTACAACCCAACACCACCAT	55.0	50.0	58.0	58.0	2259-2342 bp	1.0	87.2
rpl16-rps3_p5	CGTGCATATCGTGTCATTGC	GTACAACCCAACACCACCAT	50.0	50.0	58.0	58.0	2199-2282 bp	1.0	87.2