Hotspot

psbJ-rpoA

LSC · 89442-89862 · 421 bp

Back to Cyanotis

Pi 0.0643

MarkerSeek score 77.40

Primer available yes

Label psbJ-rpoA

Feature Metadata

label_name: psbJ-rpoA
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 27
pi: 0.0643
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-rpoA_p1	GGTTCATATTCGGGGTTGGG	ATTCCTTTTCTGCATGCGGA	55.0	45.0	58.3	58.2	547-684 bp	1.0	79.8
psbJ-rpoA_p2	GGTTCATATTCGGGGTTGGG	ATGCGGAGGAAGGGGATATT	55.0	50.0	58.3	58.3	534-671 bp	1.0	79.2
psbJ-rpoA_p3	CATATTCGGGGTTGGGTTCA	ATTCCTTTTCTGCATGCGGA	50.0	45.0	57.6	58.2	543-680 bp	1.0	78.9
psbJ-rpoA_p4	TGGTTCATATTCGGGGTTGG	ATTCCTTTTCTGCATGCGGA	50.0	45.0	57.6	58.2	548-685 bp	1.0	78.9
psbJ-rpoA_p5	GTTCATATTCGGGGTTGGGT	ATTCCTTTTCTGCATGCGGA	50.0	45.0	57.6	58.2	546-683 bp	1.0	78.9